Picosecond fluorometry in primary events of photosynthesis

Prim Ary Ionic Events in Photosynthesis

1. Fluorescence changes of umbelliferone have been used as indicator for pH changes in the outer phase of the functional membrane of photosynthesis. In combination with the repetitive pulse technique it is possible to measure the pH change which is produced during one turn-over of the molecular machinery {A pH 10-4) . The analysis of this primary pH changes gives insight in the primary ionic ev...

Early picosecond events in the photocycle of bacteriorhodopsin.

The primary processes of the photochemical cycle of light-adapted bacteriorhodopsin (BR) were studied by various experimental techniques with a time resolution of 5 x 10(-13) s. The following results were obtained. (a) After optical excitation the first excited singlet state S(1) of bacteriorhodopsin is observed via its fluorescence and absorption properties. The population of the excited singl...

The Primary Steps of Photosynthesis

Picosecond detection of an intermediate in the photochemical reaction of bacterial photosynthesis.

Preparations of photosynthetic reaction centers from Rhodopseudomonas sphaeroides were excited with flashes lasting approximately 8 psec. Immediately after the excitation, there appeared a transient state which was characterized by new absorption bands near 500 and 680 nm, by a bleaching of bands near 540, 600, 760, and 870 nm, and by a blue shift of a band near 800 nm. The transient state deca...

Evaluation of delay in Primary PCI in golden time and its relationship with major adverse cardiac events

Background: In patients with ST-segment elevation myocardial infarction (STEMI), Primary percutaneous coronary intervention (PCI) is the preferred reperfusion therapy. Timely primary PCI is essential in improving the clinical outcomes of these patients. The aim of this study was to evaluate the factors affecting balloon delay in STEMI treated patients by primary PCI and its relationship with ma...